Gujarat Technological UniversitySummer 2025 examination

GTU 3160512 Biochemical Engineering Summer 2025 Question Paper & PDF

B.E. · Chemical Engineering · Semester 6 · Subject code 3160512
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Total
70 marks
To pass
23 marks (33%)
Duration
2.5 Hours 10:30 AM – 1:00 PM
Questions
5 with internal choice
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Q.1

14 Marks
(a)
3 marks
Distinguish between bioprocess engineering and biochemical engineering.
(b)
4 marks
For animal and plant cells, mention the following differences:
1Cell wall
2Chloroplast
3Vacuoles
4Endoplasmic reticulum
(c)
7 marks

List out procedures involved in the separation and purification of intracellular enzymes. Tabulate the unit operations and their range for recovery and purification of products based on:

1Size
2Diffusivity
3Surface activity
4Density.

Q.2

14 Marks
(a)
3 marks
State the role of chelator, buffer and antifoam in the microbiological process.
(b)
4 marks
Classify protein based on its structure. What is the function of transport protein?
(c)
7 marks
Define and write chemical reactions for the synthesis of:
1Fat
2Sucrose
3Amylose
(c)
7 marks

What is the denaturation of protein? List out the five major biological functions of proteins.

Q.3

14 Marks
(a)
3 marks

List out physical methods adopted for immobilization of enzymes. What are the advantages and disadvantages of it compared to free enzyme?

(b)
4 marks
What is the effect of temperature, pH and concentration of substrate on enzyme activity?
(c)
7 marks

A substrate is converted to a product by the catalytic action of an enzyme. Assume that the Michaelis-Menten kinetic parameters for this enzyme reaction are: KM = 0.03 mol/L rmax = 13 mol/L.min

aWhat should be the size of a steady-state CSTR to convert 95% of incoming substrate CS0=10 mol/L) with a flow rate of 10L/hr?
bWhat should be the size of the reactor if a plug flow reactor is used instead of the CSTR in part (a)?
(a)
3 marks
Write assumptions involved in enzyme kinetics for the Michaelis-Menten approach.
(b)
4 marks
Differentiate between competitive and noncompetitive inhibition of enzymes.
(c)
7 marks

The initial reaction rate of hydrolysis of acetylcholine (substrate) by dog serum (source of enzyme) and obtained the following data: Substate Concentration, mol/L Initial Reaction Rate, mol/L.min 0.0032 0.111 0.0049 0.148 0.0062 0.143 0.0080 0.166 0.0095 0.200 Evaluate the Michaelis-Menten kinetic parameters by employing

athe Langmuir plot
bthe Lineweaver-Burk plot
cthe Eadie-Hofstee plot

Q.4

14 Marks
(a)
3 marks
Define the following:
1Cultivation
2Inoculation
3Sterilization
(b)
4 marks
Discuss the use of a valve and steam trap in the fermentation unit.
(c)
7 marks

Aerobic degradation of benzoic acid by a mixed culture of microorganisms can be represented by

aDetermine a, b, c, d, and e if respiratory quotient RQ = 0.9.
bDetermine the yield coefficients, YX/S and YX/O2.
(a)
3 marks
How mass transfer limitations in bioreactors are prevented? List out the variant of it.
(b)
4 marks

What are the limitations of batch reactors for microbial growth? How fed-batch reactor is advantageous for it?

(c)
7 marks

Disuses sodium sulfite method for oxygen absorption rate. A fermenter was filled with 10 L of 0.5 M sodium sulfite solution containing 0.003 M Cu++ ion and the air sparger was turned on. After 10 minutes, the airflow stopped and a 10 mL sample was taken and titrated. The concentration of the sodium sulfite in the sample was found to be 0.21 mol/L. The experiment was carried out at 25°C and 1 atm. Calculate the oxygen uptake and KLa.

Q.5

14 Marks
(a)
3 marks

What is foaming in a bioreactor? What are the adverse impacts of it? How it can be prevented?

(b)
4 marks

List out the source of probable contamination generation in a bioreactor. How can it be prevented?

(c)
7 marks

Sketch a single stirred tank reactor and write a cell and substrate mass balance. Derive an expression for dilution rate.

(a)
3 marks
List out the methods used for the separation of soluble products.
(b)
4 marks
Write about ultrafiltration and microfiltration methods.
(c)
7 marks

What is Chromatography? Discuss the various types of chromatography methods used for product separation and recovery.

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About this paper

Official Gujarat Technological University (GTU) examination paper for Biochemical Engineering (Summer 2025, B.E. · Chemical Engineering, Sem 6). Features complete 70-mark regular & remedial examination pattern, official marking distribution across all 5 questions, and direct 1-click official PDF download.

Transcribed for student exam preparation from Gujarat Technological University official examination archives. Questions, syllabus guidelines, and curriculum marking schemes remain the intellectual property of Gujarat Technological University.

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